Shenzhen Lvshiyuan Biotechnology Co., Ltd. [China]

Nitrofuran (SEM) ELISA Kit 1.Test Sensitivity: 0.1 ppb 2.Specifications: 96 wells/kit 3.MOQ: 1kit 4.ISO9001:2008

               Green Spring Nitrofuran (SEM) ELISA test kit

1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of semicarbazide (SEM) in chicken, fish, shrimp, milk, honey, whole egg, etc. The coupling antigens are pre-coated on the micro-well stripes. The SEM in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-SEM antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the SEM in it. This value is compared to the standard curve and the SEM concentration is subsequently obtained.

2. Technical specifications

Sensitivity: 0.1 ppb

Incubation Temperature: 37°C

Incubation Time:30min—30min—15min

Detection limit

Tissue, liver·················································································· 0.2 ppb

Milk, intestine, honey····································································· 0.2 ppb

Fish, shrimp (some interference) ··················································· 0.3 ppb

Recovery rate

Tissue, liver·················································································· 85±25%

Honey, milk, intestine···································································· 80±18%

Cross-reaction rate

SEM ······························································································ 100%

AHD····························································································· <0.1%

AMOZ··························································································· <0.1%

AOZ······························································································ <0.1%

3. Components

1)     Micro-well strips: 12 strips with 8 removable wells each

2)     6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb and 8.1 ppb

3)     Enzyme conjugate (12 mL) .......................................................... red cap

4)     Antibody working solution (7 mL)................................................. blue cap

5)     Substrate A solution (7 mL)........................................................ white cap

6)     Substrate B solution (7 mL)...................................................... black cap

7)     Stop solution (7 mL) .............................................................. .yellow cap

8)     20× concentrated washing buffer (40 mL).................................... white cap

9)     2× concentrated redissolving solution (50 mL).................. transparent cap

10)  2-Nitrobenzaldehyde(C7H5NO3) solution (10 mL)........................... white cap

1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of semicarbazide (SEM) in chicken, fish, shrimp, milk, honey, whole egg, etc. The coupling antigens are pre-coated on the micro-well stripes. The SEM in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-SEM antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the SEM in it. This value is compared to the standard curve and the SEM concentration is subsequently obtained.

Food Safety ELISA test kit

We are Shenzhen Lvshiyuan Biotechnology Co.,Ltd (www.lsybt.com), professional manufacturer and exporter of the most advanced Food Safety ELISA test kit(e.g. Nitrofuran ELISA kit, Clenbuterol ,Ractopamine ELISA kit), Animal Disease Diagnostic kits(e.g. Swine Foot and Mouth Disease lgG Distinguish kit,PRRSV Antibody ELISA kit),small molecule antigen and antibodies, gene recombination antigen and antibodies, etc.

Our advantages at the Diagnostic kits/ ELISA test kits field are as following:

* ISO9001:2008 certificated company

* Own a high-quality R&D team with many years of experience on bio-diagnostic field.

* Professional manufacturer of ELISA test kits, offer OEM service.

*At present, our products have been exported to over 40 countries, such as United States, Belgium, France, Korea, Germany, Singapore etc.

*Delivery time: 1-3 days after confirmation by DHL,TNT or other cheaper ways.

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Nitrofuran (SEM) ELISA rapid test kits