Aflatoxin B1 rapid test strip
Shenzhen Biokau Sci-tech Co.,ltd [China] 
1. Brief
This product is used for testing Aflatoxin B1 residue in grain, peanut, feed and edible oil sample,
suitable for on-site rapid test. Easy to operate, high sensitivity.
2. Detection limit
2.5ng/g (2.5ppb) ~50ng/g (50ppb)
3. Specifications: 50 tests/kit
4. Principle
The Aflatoxin B1 rapid test strip is an immuno-chromatographic test, using colloidal gold
immunoassay method. It relies on the competition between Aflatoxin B1 residues in the sample and?
the Aflatoxin B1 on T line, to the limited amount of dye-antibody. As a sufficient amount of drug in
the sample is presenting, the drug will saturate the antibody. Consequently, it will show an
extremely light or even invisible T line, indicating a positive result. On the other hand, if there is a
negative sample (or the amount of the drug is lower than the minimum detectable concentration), it
will generate two obvious lines in both the T and C line section.
5. Contents
1) Test card 50 pieces
2) Manual 1 piece
3) Dropper 1 piece/bag
4) Desiccant 1 piece/bag
5) Disposable gloves 3 pieces
6) Sample diluent 1 bottle
6. Material required but not provided
Equipment: Balance, centrifuge, nitrogen-drying device (or hair drier), Micropipette, Vortex
(optional).
Consumables: 50ml centrifuge tube, disposable tips, Ethyl acetate, n-hexane etc.
7. Sample preparation
7.1 Grain (Rice, corn etc.)
1) Weigh crushed grains 3±0.05 g into 50ml centrifuge tube, add 10ml Ethyl acetate, shake
vigorously by hand or Vortex for 5 min, centrifuge at 4000r/min at room temperature for 5min,
transfer 2ml supernatant into centrifuge tube, blow to dry by nitrogen or air at 56℃.
2) Add suitable quantity of sample diluent according to detection limit requirement as following
Table, blow and beat repeatedly to redisslove (Note the blow and beating of tube well), absorb 60ml
solution to test.
